O9 M1 macrophages evoke an increase in PIGR expression in MDA-MB468 breast cancer cells through interlukin-1β

Abstract Introduction The polymeric immunoglobulin receptor (PIGR) is a transmembrane protein, which transports (pIg) across the epithelial cells. High expression of PIGR in breast cancer has been reported to associate with increased 5-year survival rate. In this study, factors tumour microenvironment affected cell lines, were investigated. Method M1, M2 macrophage conditioned media (CM) and recombinant human cytokines used determine level cells secreted free secretory component (SC) evaluated by real time quantitative polymerase chain reaction Western blotting. Results M1 CM induced striking dose dependent increase mRNA MDA-MB468 cells, up 20-fold 100% CM. Interferon gamma (IFNγ) interleukin (IL)-1β also However, IL-1β was demonstrated macrophages, while IFNγ not. role from macrophages increasing confirmed IL-1 blockade, indicating that cytokine enhanced Conclusions shown present study. These imply elevated vivo may reflect polarization state associated immune Take-home Message enhances